Our affordable spectral flow cytometry instruments make complex applications possible. A New Level of Accessibility A powerful, high value system that is accessible to a wide range of users. The Cytek® Aurora offers unprecedented assay flexibility and panel complexity thanks to our unique, full spectrum optical design. N9-20017 Rev B Stain Index by Primary Excitation Laser 15 Online spectrum viewer 3. Cytek Aurora Resources Page. 2 Cytek Biosciences, Inc. 46107 Landing Parkway, Fremont, CA 94538 1-877-922-9835 [email protected] Cytek® Aurora Say Hello to a New Reality 10x Genomics Chromium. Cytek Aurora Primary Channels for Common Fluorochrome. Operation manual and fluorochrome selection guideline available. Individual components from this pdf are saved below. No changing optical filters for any fluorochrome. ... Denotes Cytek cFluor. It is equipped with 3 or 5 lasers, up to 64 fluorescence detector channels, and three scatter channels (blue FSC, blue SSC, violet SSC). The Emory Pediatrics/Winship Flow Cytomtery core recently purchased 2 addtional Cytek Aurora's (5-Lasers, matched), these were installed in Sep 2021 and Dec 2021 , one is located in the main core lab (HSRB E-362) and the second one in our lab located in Winship Buidling B on the 3rd floor (Room - B3202). Download Aurora 5 Laser Fluorochrome Guide. ... Fluorochrome selection guidelines 2. Choose the brightest fluorochrome for your least expressed protein and the dimmest fluoro-chrome for your most highly expressed protein. A2 ... enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. Contact Caisheng Lu; Location: PS17-503 Cytek® Aurora CS Brochure - Protected (PDF, 6 MB) The Cytek Aurora CS was designed to leverage the paradigm-shifting technology onboard the Cytek Aurora system and deliver the sorting capabilities you have come to expect in a high-end sorter. Aurora System The Aurora system consists of the Aurora flow cytometer and a computer workstation running SpectroFlo software for acquisition and analysis. Cytek Aurora Resources Page. The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. Laser. Cytek Aurora Users Community. The Cytek Aurora cytometer is an air-cooled, small benchtop instrument. Cytek® Aurora Fluorochrome Selection Guidelines 2 Laser 16V-14B N9-20021 Rev. 2. A Fluorochrome Signatures Dyes can be used in combination if they have unique spectrum signatures. No changing optical filters for any fluorochrome. Use any commercially available fluorochrome excited by the onboard lasers. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora system is highly flexible, intuitive and ultra-sensitive. Cytek® Aurora cFluor Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B Cytek® Aurora cFluor™ Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B Stain Index Ranking: 114 Dyes 14 Bars are color -coded by primary excitation laser(s): UV, Violet, Blue, Yellow-Green, Red SpectroFlo® QC beads are also included. Cytek® Aurora and Northern Lights Day 3. It also includes troubleshooting tips and service information. 1. Uploaded 07-01-2021 30 Color Spread Matrix with Percentages How to read this table: the fluor in the row impacts the one in the column. Cytek® Aurora cFluor™ Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R. Newly build spectral flow cytometer with a unique capability of detection over the full emission spectra. " The Cytek Aurora flow cytometer has been an amazing tool for my osteoimmunology research. This instrument was my introduction to flow cytometry completely, and I have had a pleasant experience learning to use it. With the help of my mentor and our core staff, I have run into few issues. Emission Wavelength (nm) UV Violet Blue Yellow Green Red 395 BUV395 420 BV421 440 Super Bright 436 450 Live/Dead Blue eFluor 450 480 … Cytek® Aurora cFluor Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B Cytek® Aurora 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B How to Read Full Spectrum Fluorochrome Signatures 2 Violet (16 channels) Blue (14 channels) Red (8 channels) Yellow-Green (10 channels) This dye is excited by all 5 lasers. Description. The Cytek Aurora CS was designed to leverage the paradigm-shifting technology onboard the Cytek Aurora system and deliver the sorting capabilities you have come to expect in a high-end sorter. This guide provides information on the Aurora flow cytometer, daily workflow, SpectroFlo® software features, instrument maintenance, and cytometer specifications. Cytek Aurora (with 5L similarity matrix) Configuration ... Additional optimal fluorochrome options available on FluoroFinder.com. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 colors all the way to 40 colors. The peak channel (indicated by the black bar) is in channel V11, and it has secondary emission in channels UV11, B7, YG4 and R1. Channels MFI Violet (16 channels) Blue (14 channels) Red (8 channels) Yellow Green (10 channels) This dye is excited by all 5 lasers. A2 N9-20031 Rev. With up to 5 lasers and 64 fluorescent detectors, the Cytek® Aurora can capture the entire emission spectrum from each fluorochrome. With up to three lasers (red, blue and violet), three scattering channels, and more than 30 fluorescence channels, the Aurora suits every laboratory’s needs, from simple to high complexity applications. 3. Cytek Aurora Detectors Properties. Safety Safety Symbols The Aurora is intended for research use only; not for diagnostic or therapeutic procedures. Cytek Aurora Users Community. Your fluorophore selection, type and number, will determine which instrumentation is appropriate for your panel. Meet Aurora: 2 Cytek iosciences, nc. Cytek Aurora Fluorochrome Selection Guidelines ... Cytek Aurora Fluorochrome Selection Guidelines Fluorochrome. N9-20017 Rev B Stain Index Ranking: 114 Dyes 14 Bars are color -coded by primary excitation laser(s): UV, Violet, Blue, Yellow-Green, Red. Cytek Aurora Spectra Analyzer. Member Pubs. Cytek Aurora. Members. Aurora users can find many links within the Resources page from panel design tools to helpful YouTube videos demonstrating various instrument and software capabilities. Fluorescent Dyes Brochure booklet size cp20141020 .Technical Notes on Fluorescence A = photon absorp. BioLegend Aurora Spectra Analyzer. Full Cytek Fluorochrome Selection Guideline Cytek’s guide to fluorophores on the Aurora – see expected signatures, brightness indexes, spread matrix, etc. Cytek Aurora Fluorochrome Selection Guide. Look for dyes with unique spectra and consider spread introduced by the dyes when designing multicolor panels (see slide 19). A Fluorochrome Signatures Dyes can be used in combination if they have unique spectrum signatures. In this section we provide protocols, data sheets to organize your samples, and fluorochome selection guides to assist in your experimental design. Use any commercially available fluorochrome excited by the onboard lasers. The 4 laser (16UV-16V-14B-8R) Cytek Aurora can analyze up to 54 fluorochromes, 57 parameters (FSC, 488 SSC, 405 SSC-B). 2. BioLegend Aurora Spectra Analyzer. Description. The system is intended to be used for analyzing cells in the fields of immunology, biochemistry, biology, oncology, and hematology research. Includes fluorochromes that can be used in each fluorescence channel as well as spectral overlap and spectral spreading information to aid in high parameter cytometry panel design. Maximum Accessibility A powerful, high value system that is accessible to a wide range of users. Home Instrumentation & Facilities Cytek Aurora. Use any commercially available dye excited by the lasers onboard. The Cytek Aurora is a 5 lasers spectral cytometer. When designing a multicolor panel, you should look for dyes with unique spectra and also consider spread introduced by the dyes (see slide 25). Look for dyes with unique spectra and consider spread introduced by the dyes when designing multicolor panels (see slide 27). 4 anding arkway, Fremont, CA 48 -8--8 salescytekbio.com Cyte Aurora Say ello to a ew eality In the Cytometer menu from either the QC & Setup or Acquisition module, select Fluidics Shutdown. Cytek Aurora Fluorochrome Selection Guide. Cytek Aurora Fluorochrome Selection Guidelines Fluorochrome Signatures Dyes can be used in combination as long as they have a distinct spectrum signature. The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome … CIT Intranet. The Cytek Aurora recently received a UV 355nm (20mW) laser upgrade. 405, 488 and 640nm lasers; 38 channels, 30+ unique fluorochrome signatures, 8 fluorescent protein signatures; Operation manual and fluorochrome selection guideline available. Spectral Flow Cytometry provides an alternative way to measure fluorescence emission. Cytek aurora user manual pdf free printable online However, the goal of robust panel design through selection of antigen-fluorochrome combinations while minimizing fluorescent spillover and the resulting spreading to the negative adjacent channels became even more challenging. Receive … 2 Blue Laser Excitable Dyes 3 Contact Caisheng Lu. With up to 5 lasers and 64 fluorescent detectors, the Cytek® Aurora can capture the entire emission spectrum from each fluorochrome. Brightness Index chart) Typically, PE is always the brightest fluorochrome, except in some cases where cells have particularly high autofluorescence, APC may yield the brightest fluores-cence. This makes it possible to now run 40 colors in one tube, a first for 40 fluorescence-based flow cytometry. COVID-19 Update: Learn about our efforts to help battle coronavirus. The addition of these expertly-crafted dyes to BioLegend’s portfolio provides researchers with the ability to fill spectral spaces between existing fluorophores, maximizing panel flexibility. 3. Due to the ever evolving technology and applications associated with Flow Cytometry, please discuss your … Based on this information, expect this dye to introduce spread into dyes emitting at similar wavelengths. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates. Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16UV-16V-14B-8R N9-20031 Rev. Cytek Aurora. Leave the tube of DI water on the SIP. Beckman Coulter Optima XPN … Directions. Guide to choosing fluorochromes for use on the Cytek Aurora 5 Laser Spectral Cytometer. Title: Microsoft PowerPoint - N9-20017 Rev A 5L UVVBYGR Fluor Guide.pptx Author: jshook Created Date: 10/16/2019 10:13:01 AM Similarity & complexity ... Dim Mid Bright Very Bright. N9-20021 Rev. Contrary to conventional flow cytometry which measures a limited bandwidth of the emission spectrum of each fluorochrome, spectral flow cytometry measures the entire spectral signature emitted from each of the 5 lasers. Location: PS17-514; Cytek 3L Aurora. Watch your multicolor panels grow with Spark Dyes, a family of small, synthetic fluorophores. Aurora users can find many links within the Resources page from panel design tools to helpful YouTube videos demonstrating various instrument and software capabilities. Example: 35 Fluorochrome Selection Approx. Sorry, no … Cytek’s fluorochrome guides provide examples of many dyes full spectrum signatures along with stain indices, spread information, and other useful information. Follow the prompts to load a tube with 3 mL of 10% bleach, followed by a tube with 3 mL of DI water, followed by 3 mL of 30% contrad, followed by 3 mL of DI water.
